INSIG1 antibody (Middle Region)

Catalog No. ABIN2778634

The Rabbit Polyclonal anti-INSIG1 antibody has been validated for WB and IHC. It is suitable to detect INSIG1 in samples from Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Dog, Guinea Pig, Sheep and Zebrafish (Danio rerio).

Quick Overview for INSIG1 antibody (Middle Region) (ABIN2778634)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Dog, Guinea Pig, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-INSIG1 Antibody

Binding Specificity: Middle Region

Sequence: ITIAFLATLI TQFLVYNGVY QYTSPDFLYI RSWLPCIFFS GGVTVGNIGR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against INSIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 330 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1

Background: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane prote
Alias Symbols: CL-6, MGC1405, CL6
Protein Interaction Partner: UBC, HMGCR, FAF2, SCAP, ERLIN2, ERLIN1, VCP, AMFR, PGRMC1, POR, SREBF1, ELAVL1, RNF139, INSIG2, PSMC3, PSMA7, INSIG1, VAPA, VAPB, SREBF2,
Protein Size: 330

Molecular Weight: 36 kDa

Gene ID: 3638

NCBI Accession: NM_198336, NP_938150

UniProt: A4D2M9

Pathways: ER-Nucleus Signaling